海南七星彩

海南七星彩 > 技术支持 > 关于蛋白定量的N个问题,这篇帮您一一解答!

技术支持

关于蛋白定量的N个问题,这篇帮您一一解答!
发布时间:2019-08-22   点击次数:154次

WEN1.TONGWEIDANBAIDINGLIANGCHANPIN,FEIBIAOJIDINGLIANGHEBIAOJIDINGLIANGDESHIYONGCHANGJINGYOUHECHABIE?

海南七星彩BIAOJIDINGLIANGFENXIJISHUKETONGSHIBIJIAO2-10ZUYANGPINZHIJIANDEDANBAIZUBIAODACHAYI,SHIJINNIANLAIDINGLIANGDANBAIZHIZUXUECHANGYONGDEGAOTONGLIANGSHAIXUANJISHU。ERFEIBIAOJIDINGLIANGJISHUBUXUYAOBIAOJI,DINGLIANGYANGBENSHUBUSHOUXIANZHI,QIZHONGDANBAIDIADINGLIANGFENXIJISHUBEI《Nature Methods》ZAZHIPINGWEI2015NIANZHIDEGUANZHUDEJISHUZHIYI,JUYOUDINGLIANGZHUNQUEXINGGAO、JISHUZHONGXIANGAODEYOUDIAN。

 

LIANGXIANGBIJIAOZHIXIA,FEIBIAOJIDINGLIANGDIAGENGSHIYONGYUYIXIAQINGKUANG:

1)实验组数高于10组;
2)样本来源于不同物种、不同组织或不同部位,需进行平行蛋白组定量检测;
3)需要区分样本中的“有无蛋白”;
4)追求更的定性和定量效果,避免标记和液相分组引入的实验误差;
5)需要对采集的数据进行多次重挖掘分析。

LIANGXIANGBIJIAOZHIXIA,BIAOJIDINGLIANGiTRAQ/IBTGENGSHIYONGYUYIXIAQINGKUANG:

1)实验组数低于10组(即同时比较的样本数≤10);
2)样本背景较为相似(相同物种和相同的样本类型)。

ZHEIMEKANLAI,RUGUONINDEYANGBENLIANG>10,XIANGBUSHOUXIANZHIDIHUODEGENGWEIFENGFUHEDESHUJU,DIAJIANGSHIGENGHESHIDEXUANZE

海南七星彩WEN2.DANBAIDINGLIANGYANJIUJIEGUODEDENGDAISHIJIANTAIZHANG?

MUQIANSHIMIANSHANGCHANGJIANDEDANBAIZUDINGLIANGCHANPINDEJIAOFUZHOUQICHAOGUOBANYUE,WEILEJIKEXUEYANJIUDAILAIGENGjiZHIDETIYAN,GUDUOSHENGWUTUICHULEJISUJIAOFUDEDANBAIZUDINGLIANGCHANPIN,CONGYANGBENJIANCEHEGEDAOSHUJUCHANCHU,JINXU13TIAN!TONGSHIBAOZHENGPINZHIWUSUN、JIAGEGENGYOU。

海南七星彩WEN3. RUHEBAOZHANGDANBAIDINGLIANGDEZHUNQUEXING?

CHULEZHUIQIUJIANDINGSHULIANGHEJIAGE,DINGLIANGZHUNQUECAISHIYINGDAOLI。

海南七星彩WEN3.YOUNAXIERONGYIHULVEDEGUANJIANDIANHUIYINGXIANGDANBAIDINGLIANGDEZHUNQUEXING?

海南七星彩DUIYUBIAOJIDINGLIANGDANBAIZHIZUJISHU(iTRAQ/IBT/TMT),QIYOUSHIZAIYUJIANGSUOXUBIJIAODEYANGPINBIAOJIHOUHUNZAIYIQIWUPIANHAODITONGSHIJINXINGHOUXUSHIYANHEZHIPUJIANCE,XIAOCHULEXITONGSHIYANWUCHA,SHIDEDINGLIANGKEBIXINGGENGZHIJIE。DANSHI,BIAOJIDINGLIANGJISHUDAILAIDELINGYIGEWENTISHIGONGXITUODENGYINSUDAOZHIDEDINGLIANGBIZHIYASUOXIAOYING(Nat Methods. 2011 Oct 2;8(11):937-40),YEJIUSHISHUO,SHIJIDINGLIANGJIANCEDAODECHAYIBEISHUHUIBIZHENSHICHAYIBEISHUXIAO。SUOYI,DUIYUBIAOJIDINGLIANGDANBAIZHIZUFENXIDEDAODEGANXINGQUGUANJIANDANBAI,TONGCHANGJIANYIHOUXUZUOJINYIBUDEPRMHUOMRMYANZHENGQIDINGLIANGZHUNQUEXING。

分享到:

返回列表 | 返回顶部
上一篇 : 在NGS建库过程中,如何避免常见的操作失误    下一篇 :  定量western blot:为什么需要验证抗体
网站海南七星彩 公司简介 产品中心 招聘中心 技术支持 企业动态 联系我们
上海古朵生物科技有限公司版权所有 主营:优质胎牛血清,EA36染色液配制,迪夫快速染色液
GoogleSitemap ICP备案号:沪ICP备19048203号-2 技术支持:安防展览网
在线客服